The Protective Effect of on The Reproductive System and Some Visceral Organs (Liver, Spleen) Tissues of Female Rats Exposed to High Dose of Zinc Sulphate in Drinking Water
DOI:
https://doi.org/10.56294/sctconf2024.1133Keywords:
zinc sulphate, Alchemilla vulgaris, reproductive hormonesAbstract
The study examined the effects of high concentrations of zinc sulphate in drinking water on female rats' liver, spleen, and ovaries over eight weeks. The rats were divided into five groups: control, zinc-containing, zinc-sulphate-containing, zinc-sulphate-containing, and zinc-free. As for the objectives of the study, it highlighted the protective and toxic effects of Alchemilla Vulgaris medicinal plant on female rats' liver, spleen, and ovaries tissues from high ZnSO4 concentrations in drinking water. Regarding the method of the study, it involved 30 female albino rats in a pharmacy college/university of Karbala, divided into five groups: control, treatment, treatment, and treatment, with zinc sulphate and Alchemilla vulgaris added to their drinking water. The data was analyzed using one-way ANOVA and SPSS 22.0 software, with four treatment means separated using a "protected" Duncan's analysis at a 0.05 level. The study reached some results, the most important of which are that there are changes in organ tissue, including necrosis in liver cells, congestion in spleen and ovaries, and an increase in progesterone. The study also found that the medicinal plant treated most liver diseases with low side effects, improved ovarian functions, and improved fertility. The study concluded that medicinal plant was used to treat most liver diseases better because its side effects were low. These plants had preventive effects against zinc excess and improved ovarian functions by improving the production of reproductive hormones in females.
References
1. Uddin, M. K., A review on the adsorption of heavy metals by clay minerals, with special focus on the past decade. Chemical Engineering Journal 2017, 308, 438-462.
2. Oehmen, A., Viegas, R., Velizarov, S., Reis, M. A., & Crespo, J. G., Removal of heavy metals from drinking water supplies through the ion exchange membrane bioreactor. Desalination 2006, 199(1-3), 405-407.
3. El Okle, O. S., & Lebda, M. A., Could alpha-Lipoic acid protect against sub-chronic toxicity of heavy metals mixture in japanese quails. Life Sci J 2014, (11-12), 907-917.
4. Qadir, M., Quillérou, E., Nangia, V., Murtaza, G., Singh, M., Thomas, R. J., ... & Noble, A. D., Economics of salt‐induced land degradation and restoration. In Natural resources forum 2014, November, 384 ,282-295.
5. Tennstedt, P., Peisker, D., Böttcher, C., Trampczynska, A., & Clemens, S., Phytochelatin synthesis is essential for the detoxification of excess zinc and contributes significantly to the accumulation of zinc. Plant physiology 2009,149(2), 938-948.
6. Chen, Y. W., Yang, C. Y., Huang, C. F., Hung, D. Z., Leung, Y. M., & Liu, S. H., Heavy metals, islet function and diabetes development. Islets 2009, (1-3) ,169-176.
7. Egwurugwu, J. N., Ifedi, C. U., Uchefuna, R. C., Ezeokafor, E. N., & Alagwu, E. A, Effects of zinc on male sex hormones and semen quality in rats. Nigerian Journal of Physiological Sciences 2013,28(1), 17-22.
8. Alkorta, I., Hernández-Allica, J., Becerril, J. M., Amezaga, I., Albizu, I., & Garbisu, C. . Recent findings on the phytoremediation of soils contaminated with environmentally toxic heavy metals and metalloids such as zinc, cadmium, lead, and arsenic. Reviews in Environmental Science and Biotechnology 2004, 3, 71-90.
9. Fernàndez, J., Zacchini, M., & Fleck, I. (2012). Photosynthetic and growth responses of Populus clones Eridano and I-214 submitted to elevated Zn concentrations. Journal of Geochemical Exploration 2012, 123, 77-86.
10. Singh, A., Parihar, P., Singh, R., & Prasad, S. M., An assessment to show toxic nature of beneficial trace metals: too much of good thing can be bad. International Journal of Current Multidisciplinary Studies 2016, 2, 141-144.
11. Langmead, L., & Rampton, D. S., Herbal treatment in gastrointestinal and liver disease—benefits and dangers. Alimentary pharmacology & therapeutics 2001, 15(9), 1239-1252.
12. Shinde, P., Patil, P., & Bairagi, V. ,Herbs in pregnancy and lactation: a review appraisal. International Journal of Pharmaceutical Sciences and Research 2012, 3(9), 3001.
13. Smolyakova, I. M., Andreeva, V. Y., Kalinkina, G. I., Avdeenko, S. N., & Shchetinin, P. P., Development of extraction techniques and standardization methods for a common lady’s mantle (Alchemilla vulgaris) extract. Pharmaceutical Chemistry Journal 2012, 45, 675-678.
14. Neagu, E., Paun, G., Albu, C., & Radu, G. L., Assessment of acetylcholinesterase and tyrosinase inhibitory and antioxidant activity of Alchemilla vulgaris and Filipendula ulmaria extracts. Journal of the Taiwan Institute of Chemical Engineers 2015, 52, 1-6.
15. Hamid, K. H. A., Azman, N. A. B. M., Md Shaarani, S., Zain, N., Ahmad, N., Sulaiman, A. Z., ... & Almajano Pablos, M. P. (2017). Alchemilla vulgaris and Filipendula ulmaria extracts as potential natural preservatives in beef patties.
16. Hamid, K. H. A., Azman, N. A. B. M., Md Shaarani, S., Zain, N., Ahmad, N., Sulaiman, A. Z., ... & Almajano Pablos, M. P., Alchemilla vulgaris and Filipendula ulmaria extracts as potential natural preservatives in beef patties. Malaysian Journal of Analytical Sciences 2017, 21, 986-986.
17. Cary, N., Statistical analysis system, User's guide. Statistical. Version 9. SAS. Inst. Inc. USA 2012.
18. Oğuz, A. R., & Yeltekin, A. , Metal levels in the liver, muscle, gill, intestine, and gonad of Lake Van fish (Chalcalburnus tarichi) with abnormal gonad. Biological Trace Element Research 2014,159, 219-223.
19. Georgescu, B., Georgescu, C., Dărăban, S., Bouaru, A., & Pașcalău, S.,Heavy metals acting as endocrine disrupters. Scientific Papers Animal Science and Biotechnologies 2011, 44(2), 89-89.
20. Oğuz, A. R., & Yeltekin, A. (2014). Metal levels in the liver, muscle, gill, intestine, and gonad of Lake Van fish (Chalcalburnus tarichi) with abnormal gonad. Biological Trace Element Research 2014, 159, 219-223.
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Copyright (c) 2024 Zainab Qassim Mohammed Hilo, Wafia Shaker Abdul Hussein, Dalal Abdel-Hussein Kadhim AL–Essawi (Author)
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