Platelet lysate in the treatment of burn patients, evaluation of its antimicrobial effect
DOI:
https://doi.org/10.56294/sctconf2024.699Keywords:
Dermal burns, Antimicrobial effect, platelet lysateAbstract
Introduction. Platelets can interact directly with viruses, bacteria, fungi and protozoa through proteins with direct microbicidal properties that can generate an antimicrobial effect in burns. Objective. To characterize the antimicrobial effect of platelet lysate in the treatment of burn patients. Methods. Observational, descriptive, prospective longitudinal study in patients with type A and AB dermal burns, with less than 5% of the body surface burned. Platelet concentrate production was performed using internationally established methods. Microbiological study was performed before and after the cures with platelet lysate and bacterial growth was evaluated in two subsequent cultures. Results. The mean age of the patients is 52 years and type AB burns represented 75% of the total, being more frequent in upper and lower limbs. Staphylococcus aureus and pseudomona aeruginosa were the predominant germs that grew in the burns. After the first treatment with platelet lysate, a gradual decrease in positivity to germs was observed, until in the third treatment, no more bacterial growth was observed. Conclusions. Platelet lysate has a positive antimicrobial effect, by decreasing the growth of germs isolated during the treatment of dermal burns.
References
GBD 2021 Diseases and Injuries Collaborators. Global incidence, prevalence, years lived with disability (YLDs), disability-adjusted life-years (DALYs), and healthy life expectancy (HALE) for 371 diseases and injuries in 204 countries and territories and 811 subnational locations, 1990-2021: a systematic analysis for the Global Burden of Disease Study 2021. Lancet. 2024 May 18;403(10440):2133-2161. doi: 10.1016/S0140-6736(24)00757-8. Epub 2024 Apr 17. PMID: 38642570; PMCID: PMC11122111.
NCD Risk Factor Collaboration (NCD-RisC). Worldwide trends in underweight and obesity from 1990 to 2022: a pooled analysis of 3663 population-representative studies with 222 million children, adolescents, and adults. Lancet. 2024 Mar 16;403(10431):1027-1050. doi: 10.1016/S0140-6736(23)02750-2. Epub 2024 Feb 29. PMID: 38432237; PMCID: PMC7615769.
Goh M, Du M, Peng WR, Saw PE, Chen Z. Advancing burn wound treatment: exploring hydrogel as a transdermal drug delivery system. Drug Deliv. 2024 Dec;31(1):2300945. doi: 10.1080/10717544.2023.2300945. Epub 2024 Feb 16. PMID: 38366562; PMCID: PMC10878343.
Tang XD, Qiu L, Wang F, Liu S, Lü XW, Chen XL. Safety and efficacy of waterjet debridement vs. conventional debridement in the treatment of extremely severe burns: A retrospective analysis. Burns. 2023 Dec;49(8):1926-1934. doi: 10.1016/j.burns.2023.06.004. Epub 2023 Jun 16. PMID: 37827935.
Kakudo N, Minakata T, Mitsui T, Kushi da S, Notodihardjo FZ, Kusum K. Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts. Plast Reconstr Surg. 2018;122:1352–60. https://doi.org/10.1097/PRS.0b013e3181882046
Lucarelli E, Beccheroni A, Donati D, Sangiorgi L, Cenacchi A, Del Vento AM, et al. Platelet-derived growth factors enhance proliferation of human stomal stem cells. Biomaterials. 2023;24:3095–100. https://doi.org/10.1016/s0142-9612(03)00114-5
Burnouf T, Goubran HA, Chem T_M, Ou, K_L, El-Ekiaby M, Radosevic M. Blood-derived biomaterials and platelet growth factors in regenerative medicine. Blood Rev 2013; 27: 77-89. doi: 10.1016/j.blre.2013.02.001.
Anitua E, Andía I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2014; 91:4–15. doi: 10.1160/TH03-07-0440. PMID: 14691563.
Peck MD. Epidemiology of burns throughout the world. Part I: Distribution and risk factors. Burns 2019; 37:1087-100. doi: 10.1016/j.burns.2011.06.005. Epub 2011 Jul 29. PMID: 21802856.
Citron I, Amundson J, Saluja S, Guilloux A, Jenny H, Scheffer M, et al. Assessing burn care in Brazil: an epide- miologic, cross-sectional, nationwide study. Surgery 2018; 163(5):1165-72. doi: 10.1016/j.surg.2017.11.023. Epub 2018 Feb 8. PMID: 29428152.
Ramírez-Blanco CE, Ramírez-Rivero CE, Díaz-martínez LA. Causas y sobrevida en pacientes quemados en el centro de referencia del nororiente de Colombia. Cirugía Plástica Ibero-Latinoamericana. Marzo de 2019;43(1):59-67
Koumbourlis AC. Burns injuries. Crit Care Med. 2021; 30(11 Suppl):S424-30.
Jeschke MG, van Baar ME, Choudhry MA, Chung KK, Gibran NS, Logsetty S. Burn injury. Nat Rev Dis Primers. 2020 Feb 13;6(1):11. doi: 10.1038/s41572-020-0145-5. PMID: 32054846; PMCID: PMC7224101
Moya Rosa EJ, Faces Sánchez M. Lesión por quemaduras en el adulto mayor. Archivo Médico de Camagüey. 2018; 15(5):839-47
Vierhapper MF, Lumenta DB, Beck H, Keck M, Kamolz LP, Frey M. Burn injury: a long-term analysis with review of regional differences. Ann Plast Surg. 2021;66:43—6
Burgess M, Valdera F, Varon D, Kankuri E, Nuutila K. The Immune and Regenerative Response to Burn Injury. Cells. 2022 Sep 29;11(19):3073. doi: 10.3390/cells11193073. PMID: 36231034; PMCID: PMC9563909
Moore EC, Pilcher DV, Bailey MJ, Stephens H, Cleland H. The burns evaluation and mortality study (BEAMS): predicting deaths in Australian and New Zealand burn patients admitted to intensive care with burns. J Trauma Acute Care Surg. 2019; 75(2): 298-303. doi: 10.1097/TA.0b013e318295409d. PMID: 23887563.
Carvalho G. Estudos prospectivos das vítimas de queimaduras atendidas no serviço de emergências do Hospital Regional da Asa Norte de Brasília. Rev Saúde Dist Fed. 2020;16(1/2):7-15
Church D, Elsayed S, Reid O, Winston B, Lindsay R. Burn wound infections. Clin Microbiol Rev. 2016;19:403- 34. [Medline].
Schofield CM, Murray CK, Horvath EE, Cancio LC, Kim SH, Wolf SE, et al. Correlation of culture with histopathology in fungal burn wound colonization and infection. Burns.2019;33:341-6. [Medline]. doi: 10.1016/j.burns.2006.08.040. Epub 2007 Feb 26. PMID: 17321689.
Escamilla Cardeñosa M, Domínguez Maldonado G, Córdoba Fernández A. Efficacy and safety of the use of platelet-rich plasma to manage major bunrs. Journal of tissue viability. 2019 May; 26(2):138-143.
Published
Issue
Section
License
Copyright (c) 2024 Emma Karin Salluca Vasquez, Odette Karina Montalvo Benitez, Candy Salluca Vasquez, Erika Salluca Vasquez, Jhon Michael Andy Salluca Vasquez (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
The article is distributed under the Creative Commons Attribution 4.0 License. Unless otherwise stated, associated published material is distributed under the same licence.
